› 2g4c

X-ray diffraction
3.15Å resolution

Crystal Structure of human DNA polymerase gamma accessory subunit

Released:
DOI: 10.2210/pdb2g4c/pdb
PDB entry 2g4c coloured by chain, front view.
PDB entry 2g4c coloured by chain, side view.
PDB entry 2g4c coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A novel processive mechanism for DNA synthesis revealed by structure, modeling and mutagenesis of the accessory subunit of human mitochondrial DNA polymerase.
Fan L, Kim S, Farr CL, Schaefer KT, Randolph KM, Tainer JA, Kaguni LS
J Mol Biol 358 1229-43 (2006)
PMID: 16574152

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193756 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase subunit gamma-2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 474 amino acids
Theoretical weight: 53.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9UHN1 (Residues: 26-485; Coverage: 95%)
Gene names: MTPOLB, POLG2
Sequence domains: Anticodon binding domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: P32
Unit cell:
a: 101.79Å b: 101.79Å c: 170.277Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.232 0.285
Expression system: Escherichia coli BL21

Quick links

Citations

17 review citations

DNA replication and transcription in mammalian mitochondria.
Falkenberg et al. (2007)
16 more

9 mentions without citation

A novel processive mechanism for DNA synthesis revealed by structure, modeling and mutagenesis of the accessory subunit of human mitochondrial DNA polymerase.
Fan et al. (2006)
8 more