› 2g73

X-ray diffraction
1.97Å resolution

Y104F mutant type 1 IPP isomerase complex with EIPP

Released:
DOI: 10.2210/pdb2g73/pdb
PDB entry 2g73 coloured by chain, front view.
PDB entry 2g73 coloured by chain, side view.
PDB entry 2g73 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural role for Tyr-104 in Escherichia coli isopentenyl-diphosphate isomerase: site-directed mutagenesis, enzymology, and protein crystallography.
de Ruyck J, Durisotti V, Oudjama Y, Wouters J
J Biol Chem 281 17864-9 (2006)
PMID: 16617181

Function and Biology Details

Reaction catalysed: 
Isopentenyl diphosphate = prenyl diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175139 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isopentenyl-diphosphate Delta-isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 183 amino acids
Theoretical weight: 20.63 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46822 (Residues: 1-182; Coverage: 100%)
Gene names: JW2857, b2889, idi, ygfV
Sequence domains: NUDIX domain
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase

Ligands and Environments

3 bound ligands:
Ligand MN 2 x MN
Ligand MG 2 x MG
Ligand EIP 2 x EIP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: P212121
Unit cell:
a: 68.935Å b: 71.477Å c: 91.955Å
α: 90° β: 90° γ: 90°
Expression system: Escherichia coli

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Citations

1 review citation

Molecular docking as a popular tool in drug design, an in silico travel.
de Ruyck et al. (2016)
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