› 2g7h

Solution NMR

Structure of an O6-Methylguanine DNA Methyltransferase from Methanococcus jannaschii (MJ1529)

Released:
DOI: 10.2210/pdb2g7h/pdb
PDB entry 2g7h coloured by chain, ensemble of 10 models, front view.
PDB entry 2g7h coloured by chain, ensemble of 10 models, side view.
PDB entry 2g7h coloured by chain, ensemble of 10 models, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Structural studies of MJ1529, an O6-methylguanine-DNA methyltransferase.
Roberts A, Pelton JG, Wemmer DE
Magn Reson Chem 44 Spec No S71-82 (2006)
PMID: 16826543

Function and Biology Details

Reaction catalysed: 
DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176710 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methylated-DNA--protein-cysteine methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 167 amino acids
Theoretical weight: 19.46 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58924 (Residues: 1-167; Coverage: 100%)
Gene names: MJ1529, ogt
Sequence domains: 6-O-methylguanine DNA methyltransferase, DNA binding domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing, molecular dynamics in torsion space
Expression system: Escherichia coli

Quick links

Citations

6 review citations

DNA base repair--recognition and initiation of catalysis.
Dalhus et al. (2009)
5 more

1 mention without citation

O6-alkylguanine-DNA Alkyltransferases in Microbes Living on the Edge: From Stability to Applicability.
Mattossovich et al. (2020)