› 2g83

X-ray diffraction
2.8Å resolution

Structure of activated G-alpha-i1 bound to a nucleotide-state-selective peptide: Minimal determinants for recognizing the active form of a G protein alpha subunit

Released:
DOI: 10.2210/pdb2g83/pdb
PDB entry 2g83 coloured by chain, front view.
PDB entry 2g83 coloured by chain, side view.
PDB entry 2g83 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Minimal determinants for binding activated G alpha from the structure of a G alpha(i1)-peptide dimer.
Johnston CA, Lobanova ES, Shavkunov AS, Low J, Ramer JK, Blaesius R, Fredericks Z, Willard FS, Kuhlman B, Arshavsky VY, Siderovski DP
Biochemistry 45 11390-400 (2006)
PMID: 16981699

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158956 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 313 amino acids
Theoretical weight: 35.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63096 (Residues: 33-345; Coverage: 88%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Structure domains:
KB-1753 phage display peptide Chains: C, D
Molecule details ›
Chains: C, D
Length: 11 amino acids
Theoretical weight: 1.34 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand ALF 2 x ALF
Ligand MG 2 x MG
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P3221
Unit cell:
a: 103.13Å b: 103.13Å c: 206.99Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.27 0.269 0.3
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

Receptor-mediated activation of heterotrimeric G-proteins: current structural insights.
Johnston et al. (2007)
3 more

8 mentions without citation

Regulators of G-protein signaling and their Gα substrates: promises and challenges in their use as drug discovery targets.
Kimple et al. (2011)
7 more