› 2g8y

X-ray diffraction
2.15Å resolution

The structure of a putative malate/lactate dehydrogenase from E. coli.

Released:
DOI: 10.2210/pdb2g8y/pdb
PDB entry 2g8y coloured by chain, front view.
PDB entry 2g8y coloured by chain, side view.
PDB entry 2g8y coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Cuff ME, Skarina T, Edwards A, Savchenko A, Cymborowski M, Minor W, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
(R)-3-(3,4-dihydroxyphenyl)lactate + NAD(P)(+) = 3-(3,4-dihydroxyphenyl)pyruvate + NAD(P)H
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151673 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hydroxycarboxylate dehydrogenase B Chains: A, B
Molecule details ›
Chains: A, B
Length: 385 amino acids
Theoretical weight: 42.16 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30178 (Residues: 1-361; Coverage: 100%)
Gene names: JW0786, b0801, hcxB, ybiC
Sequence domains: Malate/L-lactate dehydrogenase
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
3 bound ligands:
Ligand SO4 8 x SO4
Ligand EDO 10 x EDO
Ligand 1PE 1 x 1PE
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3221
Unit cell:
a: 139.095Å b: 139.095Å c: 151.654Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.159 0.158 0.186
Expression system: Escherichia coli BL21(DE3)

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