› 2g96

X-ray diffraction
2.9Å resolution

Crystal Structure of Visfatin/Pre-B Cell Colony Enhancing Factor 1/Nicotinamide Phosphoribosyltransferase In Complex with Niconamide Mononucleotide

Released:
DOI: 10.2210/pdb2g96/pdb
Model geometry
Fit model/data
PDB entry 2g96 coloured by chain, front view.
PDB entry 2g96 coloured by chain, side view.
PDB entry 2g96 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Crystal structure of visfatin/pre-B cell colony-enhancing factor 1/nicotinamide phosphoribosyltransferase, free and in complex with the anti-cancer agent FK-866.
Kim MK, Lee JH, Kim H, Park SJ, Kim SH, Kang GB, Lee YS, Kim JB, Kim KK, Suh SW, Eom SH
J Mol Biol 362 66-77 (2006)
PMID: 16901503

Function and Biology Details

Reaction catalysed: 
Nicotinamide D-ribonucleotide + diphosphate = nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nicotinamide phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 491 amino acids
Theoretical weight: 55.51 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q80Z29 (Residues: 1-491; Coverage: 100%)
Gene names: Nampt, Pbef1
Sequence domains:
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand NMN 2 x NMN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P212121
Unit cell:
a: 81.893Å b: 106.078Å c: 117.305Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.229 0.278
Expression system: Escherichia coli

Quick links

Citations

31 review citations

NAD+ Intermediates: The Biology and Therapeutic Potential of NMN and NR.
Yoshino et al. (2018)
30 more

2 mentions without citation

Crystallization of human nicotinamide phosphoribosyltransferase.
Takahashi et al. (2007)
1 more