› 2g9a

X-ray diffraction
2.7Å resolution

Structural basis for the specific recognition of methylated histone H3 lysine 4 by the WD-40 protein WDR5

Released:
DOI: 10.2210/pdb2g9a/pdb
PDB entry 2g9a coloured by chain, front view.
PDB entry 2g9a coloured by chain, side view.
PDB entry 2g9a coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Chai J, Han Z, Wang H, Shen Y

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158477 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
WD repeat-containing protein 5 Chain: A
Molecule details ›
Chain: A
Length: 311 amino acids
Theoretical weight: 34.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61964 (Residues: 29-334; Coverage: 92%)
Gene names: BIG3, WDR5
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Histone H3 Chain: B
Molecule details ›
Chain: B
Length: 10 amino acids
Theoretical weight: 1.2 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MLY 2 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 46.654Å b: 81.192Å c: 99.408Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 0.286
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

27 review citations

How chromatin-binding modules interpret histone modifications: lessons from professional pocket pickers.
Taverna et al. (2007)
26 more