› 2g9d

X-ray diffraction
3Å resolution

Crystal Structure of Succinylglutamate desuccinylase from Vibrio cholerae, Northeast Structural Genomics Target VcR20

Released:
DOI: 10.2210/pdb2g9d/pdb
PDB entry 2g9d coloured by chain, front view.
PDB entry 2g9d coloured by chain, side view.
PDB entry 2g9d coloured by chain, top view.
Source organism: Vibrio cholerae MO10
Entry authors: Zhou W, Jayaraman S, Forouhar F, Conover K, Rong X, Acton TB, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
N-succinyl-L-glutamate + H(2)O = succinate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192070 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Succinylglutamate desuccinylase Chain: A
Molecule details ›
Chain: A
Length: 350 amino acids
Theoretical weight: 40.78 KDa
Source organism: Vibrio cholerae MO10
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KSL4 (Residues: 1-342; Coverage: 100%)
Gene names: VC_1242, astE
Sequence domains: Succinylglutamate desuccinylase / Aspartoacylase family
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P65
Unit cell:
a: 82.934Å b: 82.934Å c: 116.665Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.241 0.241 0.272
Expression system: Escherichia coli

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