› 2g9i

X-ray diffraction
2.5Å resolution

Crystal structure of homolog of F420-0:gamma-Glutamyl Ligase from Archaeoglobus fulgidus Reveals a Novel Fold.

Released:
DOI: 10.2210/pdb2g9i/pdb
PDB entry 2g9i coloured by chain, front view.
PDB entry 2g9i coloured by chain, side view.
PDB entry 2g9i coloured by chain, top view.
Source organism: Archaeoglobus fulgidus DSM 4304
Primary publication:
Structure of an amide bond forming F(420):gamma-glutamyl ligase from Archaeoglobus fulgidus -- a member of a new family of non-ribosomal peptide synthases.
Nocek B, Evdokimova E, Proudfoot M, Kudritska M, Grochowski LL, White RH, Savchenko A, Yakunin AF, Edwards A, Joachimiak A
J Mol Biol 372 456-69 (2007)
PMID: 17669425

Function and Biology Details

Reactions catalysed: 
GTP + coenzyme F420-0 + L-glutamate = GDP + phosphate + coenzyme F420-1
GTP + coenzyme F420-1 + L-glutamate = GDP + phosphate + coenzyme gamma-F420-2
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127933 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Coenzyme F420:L-glutamate ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 27.48 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O28028 (Residues: 1-249; Coverage: 100%)
Gene names: AF_2256, cofE
Sequence domains: F420-0:Gamma-glutamyl ligase
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 100.386Å b: 100.386Å c: 92.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.19 0.255
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Physiology, Biochemistry, and Applications of F420- and Fo-Dependent Redox Reactions.
Greening et al. (2016)
1 more

4 mentions without citation

PSI-2: structural genomics to cover protein domain family space.
Dessailly et al. (2009)
3 more