› 2g9j

Solution NMR

Complex of TM1a(1-14)Zip with TM9a(251-284): a model for the polymerization domain ("overlap region") of tropomyosin, Northeast Structural Genomics Target OR9

Released:
DOI: 10.2210/pdb2g9j/pdb
PDB entry 2g9j coloured by chain, ensemble of 10 models, front view.
PDB entry 2g9j coloured by chain, ensemble of 10 models, side view.
PDB entry 2g9j coloured by chain, ensemble of 10 models, top view.
Source organisms:
Primary publication:
Solution NMR structure of the junction between tropomyosin molecules: implications for actin binding and regulation.
Greenfield NJ, Huang YJ, Swapna GV, Bhattacharya A, Rapp B, Singh A, Montelione GT, Hitchcock-DeGregori SE
J Mol Biol 364 80-96 (2006)
PMID: 16999976

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136502 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tropomyosin alpha-1 chain Chains: C, D
Molecule details ›
Chains: C, D
Length: 37 amino acids
Theoretical weight: 4.18 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P04692 (Residues: 251-284; Coverage: 12%)
Gene names: Alpha-tm, Tpm1, Tpma
General control transcription factor GCN4; Tropomyosin alpha-1 chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 33 amino acids
Theoretical weight: 3.91 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P04692 (Residues: 1-14; Coverage: 5%)
  • Canonical: P03069 (Residues: 264-281; Coverage: 6%)
Gene names: AAS101, AAS3, ARG9, Alpha-tm, GCN4, Tpm1, Tpma, YEL009C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: Initail structure was calculated with Torsion Angle Dynamics and refined with simulated annealing and included a term for explicit solvent in the refinement protocol.
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Tropomyosin-based regulation of the actin cytoskeleton in time and space.
Gunning et al. (2008)
19 more

9 mentions without citation

The role of leiomodin in actin dynamics: a new road or a secret gate.
Tolkatchev et al. (2021)
8 more