› 2g9w

X-ray diffraction
1.8Å resolution

Crystal Structure of Rv1846c, a Putative Transcriptional Regulatory Protein of Mycobacterium Tuberculosis

Released:
DOI: 10.2210/pdb2g9w/pdb
PDB entry 2g9w coloured by chain, front view.
PDB entry 2g9w coloured by chain, side view.
PDB entry 2g9w coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Genome-wide regulon and crystal structure of BlaI (Rv1846c) from Mycobacterium tuberculosis.
Sala C, Haouz A, Saul FA, Miras I, Rosenkrands I, Alzari PM, Cole ST
Mol Microbiol 71 1102-16 (2009)
PMID: 19154333

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161798 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulator BlaI Chains: A, B
Molecule details ›
Chains: A, B
Length: 138 amino acids
Theoretical weight: 15.39 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P9WMJ5 (Residues: 1-138; Coverage: 100%)
Gene names: Rv1846c, blaI
Sequence domains: Penicillinase repressor
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CL 3 x CL
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 60.58Å b: 64.33Å c: 75.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.252
Expression system: Escherichia coli BL21

Quick links

Citations

11 review citations

Division and cell envelope regulation by Ser/Thr phosphorylation: Mycobacterium shows the way.
Molle et al. (2010)
10 more

2 mentions without citation

RRE-Finder: a Genome-Mining Tool for Class-Independent RiPP Discovery.
Kloosterman et al. (2020)
1 more