› 2gaj

X-ray diffraction
1.95Å resolution

Structure of Full Length Topoisomerase I from Thermotoga maritima in monoclinic crystal form

Released:
DOI: 10.2210/pdb2gaj/pdb
PDB entry 2gaj coloured by chain, front view.
PDB entry 2gaj coloured by chain, side view.
PDB entry 2gaj coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Primary publication:
Crystal structure of full length topoisomerase I from Thermotoga maritima.
Hansen G, Harrenga A, Wieland B, Schomburg D, Reinemer P
J Mol Biol 358 1328-40 (2006)
PMID: 16600296

Function and Biology Details

Reaction catalysed: 
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155513 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 633 amino acids
Theoretical weight: 72.81 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P46799 (Residues: 1-633; Coverage: 100%)
Gene names: TM_0258, topA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 45.049Å b: 142.714Å c: 127.638Å
α: 90° β: 90.84° γ: 90°
R-values:
R R work R free
0.197 0.195 0.246
Expression system: Escherichia coli BL21(DE3)

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Citations

9 review citations

DNA topoisomerases: harnessing and constraining energy to govern chromosome topology.
Schoeffler et al. (2008)
8 more

2 mentions without citation

Phylogenomics of DNA topoisomerases: their origin and putative roles in the emergence of modern organisms.
Forterre et al. (2009)
1 more