› 2gak

X-ray diffraction
2Å resolution

X-ray crystal structure of murine leukocyte-type Core 2 b1,6-N-acetylglucosaminyltransferase (C2GnT-L)

Released:
DOI: 10.2210/pdb2gak/pdb
PDB entry 2gak coloured by chain, front view.
PDB entry 2gak coloured by chain, side view.
PDB entry 2gak coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
X-ray crystal structure of leukocyte type core 2 beta1,6-N-acetylglucosaminyltransferase. Evidence for a convergence of metal ion-independent glycosyltransferase mechanism.
Pak JE, Arnoux P, Zhou S, Sivarajah P, Satkunarajah M, Xing X, Rini JM
J Biol Chem 281 26693-701 (2006)
PMID: 16829524

Function and Biology Details

Reaction catalysed: 
UDP-N-acetyl-alpha-D-glucosamine + O(3)-(beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl)-L-seryl/threonyl-[protein] = UDP + O(3)-(beta-D-galactosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->6))-N-acetyl-alpha-D-galactosaminyl)-L-seryl/threonyl-[protein]
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170783 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 391 amino acids
Theoretical weight: 45.32 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
UniProt:
  • Canonical: Q09324 (Residues: 38-428; Coverage: 91%)
Gene name: Gcnt1
Sequence domains: Core-2/I-Branching enzyme

Ligands and Environments

1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: C2
Unit cell:
a: 126.779Å b: 79.979Å c: 104.971Å
α: 90° β: 107.7° γ: 90°
R-values:
R R work R free
0.197 0.197 0.221
Expression system: Cricetulus griseus

Quick links

Citations

13 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
12 more

2 mentions without citation

Structural Basis for the Initiation of Glycosaminoglycan Biosynthesis by Human Xylosyltransferase 1.
Briggs et al. (2018)
1 more