› 2gam

X-ray diffraction
2.7Å resolution

X-ray crystal structure of murine leukocyte-type Core 2 b1,6-N-acetylglucosaminyltransferase (C2GnT-L) in complex with Galb1,3GalNAc

Released:
DOI: 10.2210/pdb2gam/pdb
Model geometry
Fit model/data
PDB entry 2gam coloured by chain, front view.
PDB entry 2gam coloured by chain, side view.
PDB entry 2gam coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
X-ray crystal structure of leukocyte type core 2 beta1,6-N-acetylglucosaminyltransferase. Evidence for a convergence of metal ion-independent glycosyltransferase mechanism.
Pak JE, Arnoux P, Zhou S, Sivarajah P, Satkunarajah M, Xing X, Rini JM
J Biol Chem 281 26693-701 (2006)
PMID: 16829524

Function and Biology Details

Reaction catalysed: 
UDP-N-acetyl-alpha-D-glucosamine + O(3)-(beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl)-L-seryl/threonyl-[protein] = UDP + O(3)-(beta-D-galactosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->6))-N-acetyl-alpha-D-galactosaminyl)-L-seryl/threonyl-[protein]
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170783 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 391 amino acids
Theoretical weight: 45.32 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
UniProt:
  • Canonical: Q09324 (Residues: 38-428; Coverage: 91%)
Gene name: Gcnt1
Sequence domains: Core-2/I-Branching enzyme

Ligands and Environments

Carbohydrate polymer : NEW Components: NGA, GAL
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P1
Unit cell:
a: 73.498Å b: 80.585Å c: 102.325Å
α: 73.61° β: 76.26° γ: 64.56°
R-values:
R R work R free
0.217 0.217 0.274
Expression system: Cricetulus griseus

Quick links

Citations

12 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
11 more

5 mentions without citation

Emerging structural insights into glycosyltransferase-mediated synthesis of glycans.
Moremen et al. (2019)
4 more