› 2gb5

X-ray diffraction
2.3Å resolution

Crystal structure of NADH pyrophosphatase (EC 3.6.1.22) (1790429) from Escherichia coli K12 at 2.30 A resolution

Released:
DOI: 10.2210/pdb2gb5/pdb
PDB entry 2gb5 coloured by chain, front view.
PDB entry 2gb5 coloured by chain, side view.
PDB entry 2gb5 coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
NAD(H) + H(2)O = AMP + NMN(H)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152424 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD-capped RNA hydrolase NudC Chains: A, B
Molecule details ›
Chains: A, B
Length: 269 amino acids
Theoretical weight: 31.61 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P32664 (Residues: 1-257; Coverage: 100%)
Gene names: JW5548, b3996, nudC, yjaD
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21212
Unit cell:
a: 99.287Å b: 103.553Å c: 57.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.198 0.252
Expression system: Escherichia coli

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