› 2gc3

X-ray diffraction
2.1Å resolution

The crystal structure of phosphoglucose isomerase from Pyrococcus furiosus in complex with mannose 6-phosphate and zinc

Released:
DOI: 10.2210/pdb2gc3/pdb
PDB entry 2gc3 coloured by chain, front view.
PDB entry 2gc3 coloured by chain, side view.
PDB entry 2gc3 coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Evidence supporting a cis-enediol-based mechanism for Pyrococcus furiosus phosphoglucose isomerase.
Berrisford JM, Hounslow AM, Akerboom J, Hagen WR, Brouns SJ, van der Oost J, Murray IA, Michael Blackburn G, Waltho JP, Rice DW, Baker PJ
J Mol Biol 358 1353-66 (2006)
PMID: 16580686

Function and Biology Details

Reaction catalysed: 
Alpha-D-glucose 6-phosphate = beta-D-fructofuranose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160676 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-6-phosphate isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 188 amino acids
Theoretical weight: 21.38 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P83194 (Residues: 1-187; Coverage: 99%)
Gene names: PF0196, pgiA
Sequence domains: Glucose-6-phosphate isomerase (GPI)
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand M6P 2 x M6P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 70.346Å b: 74.016Å c: 74.835Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.158 0.235
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.
Bräsen et al. (2014)
11 other articles

2 mentions without citation

Structural studies of phosphoglucose isomerase from Mycobacterium tuberculosis H37Rv.
Anand et al. (2010)
1 more