› 2gc5

X-ray diffraction
1.85Å resolution

G51S mutant of L. casei FPGS

Released:
DOI: 10.2210/pdb2gc5/pdb
PDB entry 2gc5 coloured by chain, front view.
PDB entry 2gc5 coloured by chain, side view.
PDB entry 2gc5 coloured by chain, top view.
Source organism: Lacticaseibacillus casei
Primary publication:
Mutation of Gly51 to serine in the P-loop of Lactobacillus casei folylpolyglutamate synthetase abolishes activity by altering the conformation of two adjacent loops.
Smith CA, Cross JA, Bognar AL, Sun X
Acta Crystallogr D Biol Crystallogr 62 548-58 (2006)
PMID: 16627949

Function and Biology Details

Reaction catalysed: 
ATP + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147540 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Folylpolyglutamate synthase Chain: A
Molecule details ›
Chain: A
Length: 428 amino acids
Theoretical weight: 46.71 KDa
Source organism: Lacticaseibacillus casei
Expression system: Escherichia coli
UniProt:
  • Canonical: P15925 (Residues: 1-428; Coverage: 100%)
Gene name: fpgS
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand KCX 1 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 53.17Å b: 45.83Å c: 84.28Å
α: 90° β: 107.66° γ: 90°
R-values:
R R work R free
0.206 0.204 0.247
Expression system: Escherichia coli

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Citations

2 review citations

Utility of the Biosynthetic Folate Pathway for Targets in Antimicrobial Discovery.
Bourne CR. (2014)
1 more