› 2gcu

X-ray diffraction
1.48Å resolution

X-Ray Structure of Gene Product from Arabidopsis Thaliana At1g53580

Released:
DOI: 10.2210/pdb2gcu/pdb
PDB entry 2gcu coloured by chain, front view.
PDB entry 2gcu coloured by chain, side view.
PDB entry 2gcu coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structure of an ETHE1-like protein from Arabidopsis thaliana.
McCoy JG, Bingman CA, Bitto E, Holdorf MM, Makaroff CA, Phillips GN
Acta Crystallogr D Biol Crystallogr 62 964-70 (2006)
PMID: 16929096

Function and Biology Details

Reaction catalysed: 
(1a) S-sulfanylglutathione + O(2) = glutathione S-dioxide + H(+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189931 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Persulfide dioxygenase ETHE1 homolog, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 245 amino acids
Theoretical weight: 26.95 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9C8L4 (Residues: 51-294; Coverage: 83%)
Gene names: At1g53580, F22G10.9, GLY3, T3F20.11
Sequence domains: Metallo-beta-lactamase superfamily
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

3 bound ligands:
Ligand FE2 4 x FE2
Ligand SO4 1 x SO4
Ligand EDO 14 x EDO
1 modified residue:
Ligand CSD 4 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID, APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 66.607Å b: 64.469Å c: 127.877Å
α: 90° β: 97.83° γ: 90°
R-values:
R R work R free
0.178 0.176 0.204
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Chemical Biology of H2S Signaling through Persulfidation.
Filipovic et al. (2018)
3 more

10 mentions without citation

Enzymology of H2S biogenesis, decay and signaling.
Kabil et al. (2014)
9 more