› 2gcv

X-ray diffraction
2.1Å resolution

Post-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme

Released:
DOI: 10.2210/pdb2gcv/pdb
PDB entry 2gcv coloured by chain, front view.
PDB entry 2gcv coloured by chain, side view.
PDB entry 2gcv coloured by chain, top view.
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Primary publication:
Structural basis of glmS ribozyme activation by glucosamine-6-phosphate.
Klein DJ, Ferré-D'Amaré AR
Science 313 1752-6 (2006)
PMID: 16990543

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195866 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
glmS ribozyme product oligonucleotide Chain: A
Molecule details ›
Chain: A
Length: 26 nucleotides
Theoretical weight: 8.28 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Expression system: Not provided
glmS ribozyme RNA Chain: B
Molecule details ›
Chain: B
Length: 125 nucleotides
Theoretical weight: 40.6 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Expression system: Not provided
Sequence domains: glmS glucosamine-6-phosphate activated ribozyme

Ligands and Environments

2 bound ligands:
Ligand MES 2 x MES
Ligand MG 4 x MG
1 modified residue:
Ligand A23 1 x A23

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21212
Unit cell:
a: 180.55Å b: 40.46Å c: 71.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.22 0.24
Expression system: Not provided

Quick links

Citations

92 review citations

A decade of riboswitches.
Serganov et al. (2013)
91 more

9 mentions without citation

Structure and function of pseudoknots involved in gene expression control.
Peselis et al. (2014)
8 more