› 2geb

X-ray diffraction
1.7Å resolution

Crystal structure of the Thermoanaerobacter tengcongensis hypoxanthine-guanine phosphoribosyltransferase L160I mutant: insights into the inhibitor design

Released:
DOI: 10.2210/pdb2geb/pdb
PDB entry 2geb coloured by chain, front view.
PDB entry 2geb coloured by chain, side view.
PDB entry 2geb coloured by chain, top view.
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Primary publication:
Crystal structure of Thermoanaerobacter tengcongensis hypoxanthine-guanine phosphoribosyl transferase L160I mutant--insights into inhibitor design.
Chen Q, You D, Liang Y, Su X, Gu X, Luo M, Zheng X
FEBS J 274 4408-15 (2007)
PMID: 17662107

Function and Biology Details

Reaction catalysed: 
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185929 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxanthine phosphoribosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 185 amino acids
Theoretical weight: 20.77 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8R7L0 (Residues: 1-181; Coverage: 100%)
Gene names: Hpt, TTE2394
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: I222
Unit cell:
a: 52.21Å b: 88.36Å c: 93.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.226
Expression system: Escherichia coli

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Citations

2 citation in other articles

Synthesis and biological evaluation of cationic fullerene quinazolinone conjugates and their binding mode with modeled Mycobacterium tuberculosis hypoxanthine-guanine phosphoribosyltransferase enzyme.
Patel et al. (2013)
1 more