› 2gef

X-ray diffraction
2.2Å resolution

Crystal structure of a Novel viral protease with a serine/lysine catalytic dyad mechanism

Released:
DOI: 10.2210/pdb2gef/pdb
PDB entry 2gef coloured by chain, front view.
PDB entry 2gef coloured by chain, side view.
PDB entry 2gef coloured by chain, top view.
Source organism: Blotched snakehead virus
Primary publication:
Crystal structure of a novel viral protease with a serine/lysine catalytic dyad mechanism.
Feldman AR, Lee J, Delmas B, Paetzel M
J Mol Biol 358 1378-89 (2006)
PMID: 16584747

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo 24-mer
homo hexamer
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-183832 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protease VP4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 217 amino acids
Theoretical weight: 23.66 KDa
Source organism: Blotched snakehead virus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8AZM0 (Residues: 558-773; Coverage: 20%)
Sequence domains: Birnavirus VP4 protein
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: I23
Unit cell:
a: 144.07Å b: 144.07Å c: 144.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.259 0.222 0.267
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Unconventional serine proteases: variations on the catalytic Ser/His/Asp triad configuration.
Ekici et al. (2008)
2 more

5 mentions without citation

The expanding diversity of serine hydrolases.
Botos et al. (2007)
4 more