› 2gej

X-ray diffraction
2.6Å resolution

Crystal Structure of phosphatidylinositol mannosyltransferase (PimA) from Mycobacterium smegmatis in complex with GDP-Man

Released:
DOI: 10.2210/pdb2gej/pdb
PDB entry 2gej coloured by chain, front view.
PDB entry 2gej coloured by chain, side view.
PDB entry 2gej coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Primary publication:
Molecular recognition and interfacial catalysis by the essential phosphatidylinositol mannosyltransferase PimA from mycobacteria.
Guerin ME, Kordulakova J, Schaeffer F, Svetlikova Z, Buschiazzo A, Giganti D, Gicquel B, Mikusova K, Jackson M, Alzari PM
J Biol Chem 282 20705-14 (2007)
PMID: 17510062

Function and Biology Details

Reaction catalysed: 
GDP-alpha-D-mannose + 1-phosphatidyl-1D-myo-inositol = GDP + 2-O-(alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106350 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatidyl-myo-inositol mannosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 406 amino acids
Theoretical weight: 43.44 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
  • Canonical: A0QWG6 (Residues: 1-386; Coverage: 100%)
Gene names: MSMEG_2935, MSMEI_2861, pimA
Sequence domains:
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

1 bound ligand:
Ligand GDD 1 x GDD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 37.071Å b: 72.747Å c: 137.166Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.192 0.245
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Lipoarabinomannan and related glycoconjugates: structure, biogenesis and role in Mycobacterium tuberculosis physiology and host-pathogen interaction.
Mishra et al. (2011)
17 more

18 mentions without citation

Chapter 2: Biogenesis of the cell wall and other glycoconjugates of Mycobacterium tuberculosis.
Kaur et al. (2009)
17 more