› 2gfi

X-ray diffraction
2.29Å resolution

Crystal structure of the phytase from D. castellii at 2.3 A

Released:
DOI: 10.2210/pdb2gfi/pdb
PDB entry 2gfi coloured by chain, front view.
PDB entry 2gfi coloured by chain, side view.
PDB entry 2gfi coloured by chain, top view.
Source organism: Debaryomyces castellii
Primary publication:
Structure of Debaryomyces castellii CBS 2923 phytase.
Ragon M, Hoh F, Aumelas A, Chiche L, Moulin G, Boze H
Acta Crystallogr Sect F Struct Biol Cryst Commun 65 321-6 (2009)
PMID: 19342770

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106972 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phytase Chains: A, B
Molecule details ›
Chains: A, B
Length: 458 amino acids
Theoretical weight: 51.06 KDa
Source organism: Debaryomyces castellii
Expression system: Debaryomyces castellii
UniProt:
  • Canonical: A2TBB4 (Residues: 4-461; Coverage: 99%)
Gene name: phyt
Sequence domains: Histidine phosphatase superfamily (branch 2)
Structure domains: Phosphoglycerate mutase-like

Ligands and Environments

1 bound ligand:
Ligand NAG 5 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P6522
Unit cell:
a: 121.655Å b: 121.655Å c: 332.245Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.157 0.154 0.21
Expression system: Debaryomyces castellii

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Citations

1 review citation

Phytases: crystal structures, protein engineering and potential biotechnological applications.
Yao et al. (2012)
9 other articles

2 mentions without citation

Structure of Debaryomyces castellii CBS 2923 phytase.
Ragon et al. (2009)
1 more