› 2gfn

X-ray diffraction
1.9Å resolution

Crystal structure of HTH-type transcriptional regulator pksA related protein from Rhodococcus sp. RHA1

Released:
DOI: 10.2210/pdb2gfn/pdb
PDB entry 2gfn coloured by chain, front view.
PDB entry 2gfn coloured by chain, side view.
PDB entry 2gfn coloured by chain, top view.
Source organism: Rhodococcus jostii RHA1
Entry authors: Nocek B, Evdokimova E, Kagan O, Savchenko A, Edwards A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171121 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 209 amino acids
Theoretical weight: 22.6 KDa
Source organism: Rhodococcus jostii RHA1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0S7R9 (Residues: 1-209; Coverage: 100%)
Gene name: RHA1_ro04631
Sequence domains:
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 56.475Å b: 52.768Å c: 59.818Å
α: 90° β: 91.05° γ: 90°
R-values:
R R work R free
0.175 0.173 0.215
Expression system: Escherichia coli BL21(DE3)

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