› 2gge

X-ray diffraction
1.89Å resolution

Crystal Structure of Mandelate Racemase/Muconate Lactonizing Enzyme from Bacillus Subtilis complexed with MG++ at 1.8 A

Released:
DOI: 10.2210/pdb2gge/pdb
PDB entry 2gge coloured by chain, front view.
PDB entry 2gge coloured by chain, side view.
PDB entry 2gge coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Malashkevich VN, Sauder JM, Schwinn KD, Emtage S, Thompson DA, Rutter ME, Dickey M, Groshong C, Bain KT, Adams JM, Reyes C, Rooney I, Powell A, Boice A, Gheyi T, Ozyurt S, Atwell S, Wasserman SR, Burley SK, Sali A, Babbitt P, Pieper U, Gerlt JA, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126536 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative isomerase YitF Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 382 amino acids
Theoretical weight: 43.4 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O06741 (Residues: 2-371; Coverage: 100%)
Gene names: BSU10970, yitF
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 8 x MG
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 112.417Å b: 110.654Å c: 129.308Å
α: 90° β: 105.77° γ: 90°
R-values:
R R work R free
0.192 0.189 0.253
Expression system: Escherichia coli BL21

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