› 2gh1

X-ray diffraction
2.5Å resolution

Crystal Structure of the putative SAM-dependent methyltransferase BC2162 from Bacillus cereus, Northeast Structural Genomics Target BcR20.

Released:
DOI: 10.2210/pdb2gh1/pdb
PDB entry 2gh1 coloured by chain, front view.
PDB entry 2gh1 coloured by chain, side view.
PDB entry 2gh1 coloured by chain, top view.
Source organism: Bacillus cereus ATCC 14579
Entry authors: Forouhar F, Neely H, Jayaraman S, Ciao M, Xiao R, Acton TB, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182498 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyltransferase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 301 amino acids
Theoretical weight: 35.11 KDa
Source organism: Bacillus cereus ATCC 14579
Expression system: Escherichia coli
UniProt:
  • Canonical: Q81E32 (Residues: 1-293; Coverage: 100%)
Gene name: BC_2162
Sequence domains: Methyltransferase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ACT 2 x ACT
Ligand GOL 2 x GOL
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P32
Unit cell:
a: 90.889Å b: 90.889Å c: 122.319Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.195 0.227
Expression system: Escherichia coli

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