Function and Biology Details
Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- DNA-directed RNA polymerase, insert domain superfamily
- DNA-directed RNA polymerase, RpoA/D/Rpb3-type
- RNA polymerase, RBP11-like subunit
- DNA-directed RNA polymerase, alpha subunit
- RNA polymerase Rpb1, domain 5
- DNA-directed RNA polymerase, subunit 2
- DNA-directed RNA polymerase beta subunit, bacterial-type
- RNA polymerase, alpha subunit, C-terminal
22 more domains
Structure analysis Details
Assembly composition:
hetero tetramer (preferred)
Assembly name:
DNA-directed RNA polymerase (preferred)
PDBe Complex ID:
PDB-CPX-192164 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
DNA-directed RNA polymerase subunit alpha
Molecule details ›
Chains: A, B
Length: 314 amino acids
Theoretical weight: 34.83 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 314 amino acids
Theoretical weight: 34.83 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
Q9KWU8 (Residues: 1-314; Coverage: 100%)
Sequence domains:
DNA-directed RNA polymerase subunit beta
Molecule details ›
Chain: C
Length: 1119 amino acids
Theoretical weight: 124.93 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 1119 amino acids
Theoretical weight: 124.93 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: Q9KWU7 (Residues: 1-1119; Coverage: 100%)
Sequence domains:
DNA-directed RNA polymerase subunit beta'
Molecule details ›
Chain: D
Length: 1233 amino acids
Theoretical weight: 138.69 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 1233 amino acids
Theoretical weight: 138.69 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: Q9KWU6 (Residues: 1-158, 452-1524; Coverage: 81%)
Sequence domains:
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
NSLS BEAMLINE X25
Spacegroup:
P41212
Expression system: Escherichia coli BL21(DE3)
