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2ghw

X-ray diffraction
2.3Å resolution

Crystal structure of SARS spike protein receptor binding domain in complex with a neutralizing antibody, 80R

Released:
Model geometry
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Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-211326 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Spike protein S1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 203 amino acids
Theoretical weight: 22.88 KDa
Source organism: Severe acute respiratory syndrome-related coronavirus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59594 (Residues: 317-510; Coverage: 16%)
Gene names: 2, S
Sequence domains: Betacoronavirus spike glycoprotein S1, receptor binding
Structure domains: Alpha-Beta Plaits
Ig-like domain-containing protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 247 amino acids
Theoretical weight: 26.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q65ZC9 (Residues: 1-239; Coverage: 100%)
Gene name: scFv
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P21
Unit cell:
a: 47.453Å b: 175.902Å c: 67.561Å
α: 90° β: 96.55° γ: 90°
R-values:
R R work R free
0.251 0.248 0.295
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli BL21(DE3)