› 2ghy

X-ray diffraction
2.5Å resolution

Novel Crystal Form of the ColE1 Rom Protein

Released:
DOI: 10.2210/pdb2ghy/pdb
PDB entry 2ghy coloured by chain, front view.
PDB entry 2ghy coloured by chain, side view.
PDB entry 2ghy coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Novel crystal form of the ColE1 Rom protein.
Jang SB, Jeong MS, Carter RJ, Holbrook EL, Comolli LR, Holbrook SR
Acta Crystallogr D Biol Crystallogr 62 619-27 (2006)
PMID: 16699189

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136491 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNAI modulator protein Rom; Regulatory protein rop Chains: A, B
Molecule details ›
Chains: A, B
Length: 63 amino acids
Theoretical weight: 7.24 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7BLB4 (Residues: 1-63; Coverage: 100%)
  • Canonical: P03051 (Residues: 1-63; Coverage: 100%)
Gene names: A8502_004534, BGM66_004730, BvCmsKKP061_05282, D9C02_26580, D9D43_28005, D9J61_24500, DD762_27200, DTL43_26080, EN85_004810, F7N46_25900, F9413_23695, FIJ20_25470, FPS11_28565, FZU14_27260, GAJ12_26360, GOP25_27045, GQE86_25290, GQW07_24325, GRO95_24330, HEP34_005012, HIE29_005411, HJQ60_005437, HJQ60_005540, HMV95_24160, HUS41_pIII0001, HV209_29180, HVY77_28380, HVY77_28445, I6H00_27315, NCTC10082_05556, NCTC9045_06076, QDW62_26105, RCS83_PIII0002, SAMEA3472112_05909, SAMEA3752557_05352, SAMEA3752557_05360, rom, rop, rop_1, rop_2, rop_3
Sequence domains: Rop protein
Structure domains: Helix Hairpins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 97.77Å b: 39.11Å c: 26.7Å
α: 90° β: 96.16° γ: 90°
R-values:
R R work R free
0.179 0.179 0.263
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Proteins that interact with bacterial small RNA regulators.
Pichon et al. (2007)
5 other articles