› 2gis

X-ray diffraction
2.9Å resolution

Structure of the S-adenosylmethionine riboswitch mRNA regulatory element

Released:
DOI: 10.2210/pdb2gis/pdb
PDB entry 2gis coloured by chain, front view.
PDB entry 2gis coloured by chain, side view.
PDB entry 2gis coloured by chain, top view.
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Primary publication:
Structure of the S-adenosylmethionine riboswitch regulatory mRNA element.
Montange RK, Batey RT
Nature 441 1172-5 (2006)
PMID: 16810258

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195849 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
SAM-I riboswitch Chain: A
Molecule details ›
Chain: A
Length: 94 nucleotides
Theoretical weight: 30.55 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Expression system: Not provided
Sequence domains: SAM riboswitch (S box leader)

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand IRI 4 x IRI
Ligand SAM 1 x SAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P43212
Unit cell:
a: 62.901Å b: 62.901Å c: 158.967Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.266 0.266 0.289
Expression system: Not provided

Quick links

Citations

59 review citations

X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution.
Putnam et al. (2007)
58 more

74 mentions without citation

The structural and functional diversity of metabolite-binding riboswitches.
Roth et al. (2009)
73 more