› 2gix

X-ray diffraction
2.02Å resolution

Cytoplasmic Domain Structure of Kir2.1 containing Andersen's Mutation R218Q and Rescue Mutation T309K

Released:
DOI: 10.2210/pdb2gix/pdb
PDB entry 2gix coloured by chain, front view.
PDB entry 2gix coloured by chain, side view.
PDB entry 2gix coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Andersen's syndrome mutation effects on the structure and assembly of the cytoplasmic domains of Kir2.1.
Pegan S, Arrabit C, Slesinger PA, Choe S
Biochemistry 45 8599-606 (2006)
PMID: 16834334

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-152949 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inward rectifier potassium channel 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 208 amino acids
Theoretical weight: 23.91 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P35561 (Residues: 187-371; Coverage: 43%)
Gene names: Irk1, Kcnj2
Sequence domains: Inward rectifier potassium channel C-terminal domain
Structure domains: G protein-activated inward rectifier potassium channel 1

Ligands and Environments

2 bound ligands:
Ligand K 1 x K
Ligand MPD 4 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2
Unit cell:
a: 140.674Å b: 98.876Å c: 98.094Å
α: 90° β: 130.68° γ: 90°
R-values:
R R work R free
0.191 0.177 0.229
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Emerging roles for G protein-gated inwardly rectifying potassium (GIRK) channels in health and disease.
Lüscher et al. (2010)
12 more

5 mentions without citation

A discrete alcohol pocket involved in GIRK channel activation.
Aryal et al. (2009)
4 more