› 2gja

X-ray diffraction
1.85Å resolution

Structure of the MnmE G-domain in complex with GDP*AlF4-, Mg2+ and NH4+

Released:
DOI: 10.2210/pdb2gja/pdb
PDB entry 2gja coloured by chain, front view.
PDB entry 2gja coloured by chain, side view.
PDB entry 2gja coloured by chain, top view.
Source organism: Escherichia coli BL21(DE3)
Primary publication:
Dimerisation-dependent GTPase reaction of MnmE: how potassium acts as GTPase-activating element.
Scrima A, Wittinghofer A
EMBO J 25 2940-51 (2006)
PMID: 16763562

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150493 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA modification GTPase MnmE Chains: A, B
Molecule details ›
Chains: A, B
Length: 172 amino acids
Theoretical weight: 18.62 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25522 (Residues: 216-384; Coverage: 37%)
Gene names: JW3684, b3706, mnmE, thdF, trmE
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand ALF 2 x ALF
Ligand MG 2 x MG
Ligand NH4 2 x NH4
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2221
Unit cell:
a: 71.28Å b: 90.65Å c: 138.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.228
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Structure-function relationships of the G domain, a canonical switch motif.
Wittinghofer et al. (2011)
14 more

6 mentions without citation

Dimerisation-dependent GTPase reaction of MnmE: how potassium acts as GTPase-activating element.
Scrima et al. (2006)
5 more