› 2gll

X-ray diffraction
2.2Å resolution

Crystal structure of (3R)-Hydroxyacyl-Acyl Carrier Protein Dehydratase(FabZ) from Helicobacter pylori

Released:
DOI: 10.2210/pdb2gll/pdb
PDB entry 2gll coloured by chain, front view.
PDB entry 2gll coloured by chain, side view.
PDB entry 2gll coloured by chain, top view.
Source organism: Helicobacter pylori SS1
Primary publication:
Structural basis for catalytic and inhibitory mechanisms of beta-hydroxyacyl-acyl carrier protein dehydratase (FabZ).
Zhang L, Liu W, Hu T, Du L, Luo C, Chen K, Shen X, Jiang H
J Biol Chem 283 5370-9 (2008)
PMID: 18093984

Function and Biology Details

Reaction catalysed: 
A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127805 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 171 amino acids
Theoretical weight: 19.61 KDa
Source organism: Helicobacter pylori SS1
Expression system: Escherichia coli
UniProt:
  • Canonical: O25928 (Residues: 1-159; Coverage: 100%)
Gene names: HP_1376, fabZ
Sequence domains: FabA-like domain
Structure domains: Hotdog Thioesterase

Ligands and Environments

2 bound ligands:
Ligand CL 6 x CL
Ligand BEN 8 x BEN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 73.857Å b: 100.585Å c: 185.752Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.255
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Gates of enzymes.
Gora et al. (2013)
8 more

5 mentions without citation

Active site comparisons and catalytic mechanisms of the hot dog superfamily.
Labonte et al. (2013)
4 more