› 2gm4

X-ray diffraction
3.5Å resolution

An activated, tetrameric gamma-delta resolvase: Hin chimaera bound to cleaved DNA

Released:
DOI: 10.2210/pdb2gm4/pdb
PDB entry 2gm4 coloured by chain, front view.
PDB entry 2gm4 coloured by chain, side view.
PDB entry 2gm4 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Implications of structures of synaptic tetramers of gamma delta resolvase for the mechanism of recombination.
Kamtekar S, Ho RS, Cocco MJ, Li W, Wenwieser SV, Boocock MR, Grindley ND, Steitz TA
Proc Natl Acad Sci U S A 103 10642-7 (2006)
PMID: 16807292

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115306 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transposon gamma-delta resolvase Chains: A, B
Molecule details ›
Chains: A, B
Length: 183 amino acids
Theoretical weight: 20.34 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P03012 (Residues: 1-183; Coverage: 100%)
Gene names: ECOK12F009, tnpR
Sequence domains:
Structure domains:
5'-D(*CP*AP*GP*TP*GP*TP*CP*CP*GP*AP*TP*AP*AP*TP*TP*TP*AP*TP*AP*AP*A)-3' Chains: I, J, X, Z
Molecule details ›
Chains: I, J, X, Z
Length: 21 nucleotides
Theoretical weight: 6.45 KDa
Source organism: Escherichia coli
Expression system: Not provided
5'-D(*TP*TP*AP*TP*CP*GP*GP*AP*CP*AP*CP*TP*G)-3' Chains: K, Y
Molecule details ›
Chains: K, Y
Length: 13 nucleotides
Theoretical weight: 3.97 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P21212
Unit cell:
a: 110.138Å b: 137.687Å c: 83.473Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.282 0.28 0.323
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Serine recombinases as tools for genome engineering.
Brown et al. (2011)
2 more

10 mentions without citation

Topoisomerases and site-specific recombinases: similarities in structure and mechanism.
Yang W. (2010)
9 more