› 2gm5

X-ray diffraction
2.1Å resolution

An activated, truncated gamma-delta resolvase tetramer

Released:
DOI: 10.2210/pdb2gm5/pdb
PDB entry 2gm5 coloured by chain, front view.
PDB entry 2gm5 coloured by chain, side view.
PDB entry 2gm5 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Implications of structures of synaptic tetramers of gamma delta resolvase for the mechanism of recombination.
Kamtekar S, Ho RS, Cocco MJ, Li W, Wenwieser SV, Boocock MR, Grindley ND, Steitz TA
Proc Natl Acad Sci U S A 103 10642-7 (2006)
PMID: 16807292

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136459 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transposon gamma-delta resolvase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 139 amino acids
Theoretical weight: 15.69 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P03012 (Residues: 2-134; Coverage: 73%)
Gene names: ECOK12F009, tnpR
Sequence domains: Resolvase, N terminal domain
Structure domains: Resolvase, N-terminal catalytic domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 53.331Å b: 98.476Å c: 100.408Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.215 0.255
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Serine recombinases as tools for genome engineering.
Brown et al. (2011)
2 more

12 mentions without citation

Topoisomerases and site-specific recombinases: similarities in structure and mechanism.
Yang W. (2010)
11 more