› 2gok

X-ray diffraction
1.87Å resolution

Crystal structure of the imidazolonepropionase from Agrobacterium tumefaciens at 1.87 A resolution

Released:
DOI: 10.2210/pdb2gok/pdb
PDB entry 2gok coloured by chain, front view.
PDB entry 2gok coloured by chain, side view.
PDB entry 2gok coloured by chain, top view.
Source organism: Agrobacterium fabrum str. C58
Primary publication:
X-ray structure of imidazolonepropionase from Agrobacterium tumefaciens at 1.87 A resolution.
Tyagi R, Kumaran D, Burley SK, Swaminathan S
Proteins 69 652-8 (2007)
PMID: 17640072

Function and Biology Details

Reaction catalysed: 
(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H(2)O = N-formimidoyl-L-glutamate + H(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186508 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Imidazolonepropionase Chains: A, B
Molecule details ›
Chains: A, B
Length: 429 amino acids
Theoretical weight: 45.66 KDa
Source organism: Agrobacterium fabrum str. C58
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U8Z6 (Residues: 2-419; Coverage: 100%)
Gene names: AGR_L_1824, Atu3934, hutI
Sequence domains: Amidohydrolase family
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand FE 2 x FE
Ligand MG 2 x MG
Ligand CL 1 x CL
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2
Unit cell:
a: 140.754Å b: 63.63Å c: 103.716Å
α: 90° β: 111.93° γ: 90°
R-values:
R R work R free
0.218 0.207 0.231
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Functional identification and structure determination of two novel prolidases from cog1228 in the amidohydrolase superfamily .
Xiang et al. (2010)

6 mentions without citation

PSI-2: structural genomics to cover protein domain family space.
Dessailly et al. (2009)
5 more