› 2gpy

X-ray diffraction
1.9Å resolution

Crystal structure of putative O-methyltransferase from Bacillus halodurans

Released:
DOI: 10.2210/pdb2gpy/pdb
PDB entry 2gpy coloured by chain, front view.
PDB entry 2gpy coloured by chain, side view.
PDB entry 2gpy coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans C-125
Entry authors: Ramagopal UA, Patskovsky YV, Almo SC, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191844 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA 5-hydroxyuridine methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 233 amino acids
Theoretical weight: 27.36 KDa
Source organism: Alkalihalobacillus halodurans C-125
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KDE1 (Residues: 2-223; Coverage: 100%)
Gene names: BH1272, trmR
Sequence domains: O-methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

2 bound ligands:
Ligand ZN 9 x ZN
Ligand MG 1 x MG
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A, NSLS BEAMLINE X9A
Spacegroup: P212121
Unit cell:
a: 50.567Å b: 62.807Å c: 137.746Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.248
Expression system: Escherichia coli

Quick links