› 2gqr

X-ray diffraction
2Å resolution

SAICAR Synthetase Complexed with ADP-Mg2+

Released:
DOI: 10.2210/pdb2gqr/pdb
PDB entry 2gqr coloured by chain, front view.
PDB entry 2gqr coloured by chain, side view.
PDB entry 2gqr coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Nucleotide complexes of Escherichia coli phosphoribosylaminoimidazole succinocarboxamide synthetase.
Ginder ND, Binkowski DJ, Fromm HJ, Honzatko RB
J Biol Chem 281 20680-20688 (2006)
PMID: 16687397

Function and Biology Details

Reaction catalysed: 
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141585 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylaminoimidazole-succinocarboxamide synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 237 amino acids
Theoretical weight: 27.45 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7D7 (Residues: 1-237; Coverage: 100%)
Gene names: JW2461, b2476, purC
Sequence domains: SAICAR synthetase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ADP 2 x ADP
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P212121
Unit cell:
a: 59.29Å b: 67.16Å c: 148.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.204 0.239
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structural biology of the purine biosynthetic pathway.
Zhang et al. (2008)
1 more

2 mentions without citation

AcsD catalyzes enantioselective citrate desymmetrization in siderophore biosynthesis.
Schmelz et al. (2009)
1 more