› 2gqu

X-ray diffraction
1.6Å resolution

Crystal Structure of UDP-N-Acetylenolpyruvylglucosamine Reductase (MurB) from Thermus caldophilus

Released:
DOI: 10.2210/pdb2gqu/pdb
PDB entry 2gqu coloured by chain, front view.
PDB entry 2gqu coloured by chain, side view.
PDB entry 2gqu coloured by chain, top view.
Source organism: Thermus caldophilus
Primary publication:
Crystal structure of UDP-N-acetylenolpyruvylglucosamine reductase (MurB) from Thermus caldophilus.
Kim MK, Cho MK, Song HE, Kim D, Park BH, Lee JH, Kang GB, Kim SH, Im YJ, Lee DS, Eom SH
Proteins 66 751-4 (2007)
PMID: 17120230

Function and Biology Details

Reaction catalysed: 
UDP-N-acetyl-alpha-D-muramate + NADP(+) = UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178013 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylenolpyruvoylglucosamine reductase Chain: A
Molecule details ›
Chain: A
Length: 268 amino acids
Theoretical weight: 29.63 KDa
Source organism: Thermus caldophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SJC8 (Residues: 1-265; Coverage: 100%)
Gene names: TTHA1086, murB
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
1 bound ligand:
Ligand EPU 1 x EPU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P1
Unit cell:
a: 29.817Å b: 46.85Å c: 48.593Å
α: 65.26° β: 76.13° γ: 84.01°
R-values:
R R work R free
0.172 0.17 0.223
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Cytoplasmic steps of peptidoglycan biosynthesis.
Barreteau et al. (2008)
1 more

1 mention without citation

LucY: A Versatile New Fluorescent Reporter Protein.
Auldridge et al. (2015)