› 2gs4

X-ray diffraction
2Å resolution

The crystal structure of the E.coli stress protein YciF.

Released:
DOI: 10.2210/pdb2gs4/pdb
PDB entry 2gs4 coloured by chain, front view.
PDB entry 2gs4 coloured by chain, side view.
PDB entry 2gs4 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The crystal structure of the E. coli stress protein YciF.
Hindupur A, Liu D, Zhao Y, Bellamy HD, White MA, Fox RO
Protein Sci 15 2605-11 (2006)
PMID: 17001035

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-149215 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein YciF Chains: A, B
Molecule details ›
Chains: A, B
Length: 166 amino acids
Theoretical weight: 18.81 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli str. K-12 substr. DH10B
UniProt:
  • Canonical: P21362 (Residues: 1-166; Coverage: 100%)
Gene names: JW1250, b1258, yciF
Sequence domains: Domain of unknown function (DUF892)
Structure domains: Ferritin

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: CAMD BEAMLINE GCPCC
Spacegroup: R3
Unit cell:
a: 79.87Å b: 79.87Å c: 131.24Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.183 0.224
Expression system: Escherichia coli str. K-12 substr. DH10B

Quick links

Citations

13 citation in other articles

An extracytoplasmic function sigma factor acts as a general stress response regulator in Sinorhizobium meliloti.
Sauviac et al. (2007)
12 more

3 mentions without citation

Heterologous expression of mycobacterial Esx complexes in Escherichia coli for structural studies is facilitated by the use of maltose binding protein fusions.
Arbing et al. (2013)
2 more