› 2gsh

X-ray diffraction
2.39Å resolution

Crystal structure of L-rhamnonate dehydratase from Salmonella typhimurium

Released:
DOI: 10.2210/pdb2gsh/pdb
PDB entry 2gsh coloured by chain, front view.
PDB entry 2gsh coloured by chain, side view.
PDB entry 2gsh coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Entry authors: Patskovsky Y, Malashkevich VN, Sauder JM, Dickey M, Adams JM, Wasserman SR, Gerlt J, Almo SC, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
L-rhamnonate = 2-dehydro-3-deoxy-L-rhamnonate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187303 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-rhamnonate dehydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 414 amino acids
Theoretical weight: 45.84 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8ZNF9 (Residues: 2-405; Coverage: 100%)
Gene names: STM2291, rhmD, yfaW
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P23
Unit cell:
a: 135.145Å b: 135.145Å c: 135.145Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.194 0.241
Expression system: Escherichia coli

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