› 2gsw

X-ray diffraction
2.92Å resolution

Crystal Structure of the Putative NADPH-dependent Azobenzene FMN-Reductase YhdA from Bacillus subtilis, Northeast Structural Genomics Target SR135

Released:
DOI: 10.2210/pdb2gsw/pdb
PDB entry 2gsw coloured by chain, front view.
PDB entry 2gsw coloured by chain, side view.
PDB entry 2gsw coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Entry authors: Forouhar F, Hussain M, Jayaraman S, Shen J, Cooper B, Cunningham K, Janjua H, Ma L-C, Xiao R, Acton TB, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126590 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FMN-dependent NADPH-azoreductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 182 amino acids
Theoretical weight: 20.28 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: O07529 (Residues: 1-174; Coverage: 100%)
Gene names: BSU09340, azr, yhdA
Sequence domains: NADPH-dependent FMN reductase
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand FMN 4 x FMN
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 52.444Å b: 99.296Å c: 69.436Å
α: 90° β: 103.34° γ: 90°
R-values:
R R work R free
0.262 0.26 0.291
Expression system: Escherichia coli

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