› 2guc

X-ray diffraction
1.79Å resolution

Crystal structure of a complex of griffithsin with mannose at 1.78 A resolution.

Released:
DOI: 10.2210/pdb2guc/pdb
PDB entry 2guc coloured by chain, front view.
PDB entry 2guc coloured by chain, side view.
PDB entry 2guc coloured by chain, top view.
Source organism: Griffithsia
Primary publication:
Domain-swapped structure of the potent antiviral protein griffithsin and its mode of carbohydrate binding.
Ziółkowska NE, O'Keefe BR, Mori T, Zhu C, Giomarelli B, Vojdani F, Palmer KE, McMahon JB, Wlodawer A
Structure 14 1127-35 (2006)
PMID: 16843894

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160913 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Griffithsin Chains: A, B
Molecule details ›
Chains: A, B
Length: 122 amino acids
Theoretical weight: 12.73 KDa
Source organism: Griffithsia
Expression system: Nicotiana benthamiana
UniProt:
  • Canonical: P84801 (Residues: 1-121; Coverage: 100%)
Sequence domains: Jacalin-like lectin domain
Structure domains: Jacalin-like lectin domain

Ligands and Environments

3 bound ligands:
Ligand MAN 7 x MAN
Ligand EDO 1 x EDO
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 53.77Å b: 39.46Å c: 57.24Å
α: 90° β: 91° γ: 90°
R-values:
R R work R free
0.151 0.151 0.189
Expression system: Nicotiana benthamiana

Quick links

Citations

27 review citations

Protein acrobatics in pairs--dimerization via domain swapping.
Gronenborn AM. (2009)
26 more

6 mentions without citation

Mannose-Specific Lectins from Marine Algae: Diverse Structural Scaffolds Associated to Common Virucidal and Anti-Cancer Properties.
Barre et al. (2019)
5 more