› 2guf

X-ray diffraction
1.95Å resolution

In meso crystal structure of the cobalamin transporter, BtuB

Released:
DOI: 10.2210/pdb2guf/pdb
PDB entry 2guf coloured by chain, front view.
PDB entry 2guf coloured by chain, side view.
PDB entry 2guf coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
In meso structure of the cobalamin transporter, BtuB, at 1.95 A resolution.
Cherezov V, Yamashita E, Liu W, Zhalnina M, Cramer WA, Caffrey M
J Mol Biol 364 716-34 (2006)
PMID: 17028020

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138877 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vitamin B12 transporter BtuB Chain: A
Molecule details ›
Chain: A
Length: 594 amino acids
Theoretical weight: 66.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P06129 (Residues: 21-614; Coverage: 100%)
Gene names: JW3938, b3966, bfe, btuB, cer, dcrC
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MPG 11 x MPG
Ligand FMT 1 x FMT
Ligand MPD 4 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P212121
Unit cell:
a: 72.61Å b: 80.877Å c: 118.681Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.225
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Colicin biology.
Cascales et al. (2007)
10 more

15 mentions without citation

TonB-dependent transporters: regulation, structure, and function.
Noinaj et al. (2010)
14 more