› 2guh

X-ray diffraction
1.52Å resolution

Crystal Structure of the Putative TetR-family Transcriptional Regulator from Rhodococcus sp. RHA1

Released:
DOI: 10.2210/pdb2guh/pdb
PDB entry 2guh coloured by chain, front view.
PDB entry 2guh coloured by chain, side view.
PDB entry 2guh coloured by chain, top view.
Source organism: Rhodococcus jostii RHA1
Entry authors: Kim Y, Joachimiak A, Skarina T, Zheng H, Edwards A, Savchenko A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171127 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 214 amino acids
Theoretical weight: 24.01 KDa
Source organism: Rhodococcus jostii RHA1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0S8Y3 (Residues: 1-190; Coverage: 100%)
Gene name: RHA1_ro04212
Sequence domains:
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

2 bound ligands:
Ligand MG 3 x MG
Ligand EDO 4 x EDO
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 42.259Å b: 72.13Å c: 133.875Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.188
Expression system: Escherichia coli BL21(DE3)

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