› 2gup

X-ray diffraction
2.01Å resolution

Structural Genomics, the crystal structure of a ROK family protein from Streptococcus pneumoniae TIGR4 in complex with sucrose

Released:
DOI: 10.2210/pdb2gup/pdb
PDB entry 2gup coloured by chain, front view.
PDB entry 2gup coloured by chain, side view.
PDB entry 2gup coloured by chain, top view.
Source organism: Streptococcus pneumoniae TIGR4
Entry authors: Tan K, Li H, Abdullah J, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101507 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
ROK family protein Chain: A
Molecule details ›
Chain: A
Length: 292 amino acids
Theoretical weight: 32 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A0H2USG4 (Residues: 1-289; Coverage: 100%)
Gene name: SP_2142
Sequence domains: ROK family
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
Carbohydrate polymer
1 bound ligand:
Ligand TRS 1 x TRS
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 54.678Å b: 114.665Å c: 117.677Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.228
Expression system: Escherichia coli BL21

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