› 2guz

X-ray diffraction
2Å resolution

Structure of the Tim14-Tim16 complex of the mitochondrial protein import motor

Released:
DOI: 10.2210/pdb2guz/pdb
PDB entry 2guz coloured by chain, front view.
PDB entry 2guz coloured by chain, side view.
PDB entry 2guz coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure and function of Tim14 and Tim16, the J and J-like components of the mitochondrial protein import motor.
Mokranjac D, Bourenkov G, Hell K, Neupert W, Groll M
EMBO J 25 4675-85 (2006)
PMID: 16977310

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154919 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitochondrial import inner membrane translocase subunit TIM14 Chains: A, C, E, G, I, K, M, O
Molecule details ›
Chains: A, C, E, G, I, K, M, O
Length: 71 amino acids
Theoretical weight: 7.98 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07914 (Residues: 99-168; Coverage: 42%)
Gene names: PAM18, TIM14, YLR008C
Structure domains: DnaJ domain
Mitochondrial import inner membrane translocase subunit TIM16 Chains: B, D, F, H, J, L, N, P
Molecule details ›
Chains: B, D, F, H, J, L, N, P
Length: 65 amino acids
Theoretical weight: 7.76 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P42949 (Residues: 54-117; Coverage: 43%)
Gene names: J0822, PAM16, TIM16, YJL104W
Sequence domains: Pam16
Structure domains: DnaJ domain

Ligands and Environments

1 bound ligand:
Ligand FLC 4 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P212121
Unit cell:
a: 111.591Å b: 114.441Å c: 162.191Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.205 0.253
Expression system: Escherichia coli

Quick links

Citations

27 review citations

Translocation of proteins into mitochondria.
Neupert et al. (2007)
26 more

6 mentions without citation

Structure and function of Tim14 and Tim16, the J and J-like components of the mitochondrial protein import motor.
Mokranjac et al. (2006)
5 more