› 2gvc

X-ray diffraction
2.22Å resolution

Crystal structure of flavin-containing monooxygenase (FMO)from S.pombe and substrate (methimazole) complex

Released:
DOI: 10.2210/pdb2gvc/pdb
PDB entry 2gvc coloured by chain, front view.
PDB entry 2gvc coloured by chain, side view.
PDB entry 2gvc coloured by chain, top view.
Source organism: Schizosaccharomyces pombe
Primary publication:
Mechanism of action of a flavin-containing monooxygenase.
Eswaramoorthy S, Bonanno JB, Burley SK, Swaminathan S
Proc Natl Acad Sci U S A 103 9832-7 (2006)
PMID: 16777962

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190813 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiol-specific monooxygenase Chains: A, B, D, E
Molecule details ›
Chains: A, B, D, E
Length: 447 amino acids
Theoretical weight: 50.18 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HFE4 (Residues: 1-447; Coverage: 100%)
Gene names: SPBP16F5.08c, fmo1
Sequence domains:
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments

4 bound ligands:
Ligand PEO 4 x PEO
Ligand FAD 4 x FAD
Ligand MMZ 4 x MMZ
Ligand GOL 4 x GOL
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P1
Unit cell:
a: 72.7Å b: 84.45Å c: 113.51Å
α: 107.73° β: 90.76° γ: 106.5°
R-values:
R R work R free
0.23 0.23 0.264
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Flavoenzymes.
Joosten et al. (2007)
13 more

5 mentions without citation

Mechanism of action of a flavin-containing monooxygenase.
Eswaramoorthy et al. (2006)
4 more