› 2gw3

X-ray diffraction
1.4Å resolution

Crystal structure of stony coral fluorescent protein Kaede, green form

Released:
DOI: 10.2210/pdb2gw3/pdb
PDB entry 2gw3 coloured by chain, front view.
PDB entry 2gw3 coloured by chain, side view.
PDB entry 2gw3 coloured by chain, top view.
Source organism: Trachyphyllia geoffroyi
Primary publication:
Crystallographic evidence for water-assisted photo-induced peptide cleavage in the stony coral fluorescent protein Kaede.
Hayashi I, Mizuno H, Tong KI, Furuta T, Tanaka F, Yoshimura M, Miyawaki A, Ikura M
J Mol Biol 372 918-926 (2007)
PMID: 17692334

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184700 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kaede Chains: A, B
Molecule details ›
Chains: A, B
Length: 225 amino acids
Theoretical weight: 25.86 KDa
Source organism: Trachyphyllia geoffroyi
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8I6J8 (Residues: 1-225; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand NI 4 x NI
1 modified residue:
Ligand CR8 2 x CR8

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: C2
Unit cell:
a: 132.496Å b: 80.476Å c: 53.528Å
α: 90° β: 113.82° γ: 90°
R-values:
R R work R free
0.197 0.197 0.214
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Fluorescent proteins for live-cell imaging with super-resolution.
Nienhaus et al. (2014)
15 more

5 mentions without citation

Monomerization of the photoconvertible fluorescent protein SAASoti by rational mutagenesis of single amino acids.
Solovyev et al. (2018)
4 more