› 2gwa

X-ray diffraction
1.75Å resolution

Crystal Structure of a Complex Formed Between the DNA Holliday Junction and a Bis-Acridine Molecule.

Released:
DOI: 10.2210/pdb2gwa/pdb
PDB entry 2gwa coloured by chain, front view.
PDB entry 2gwa coloured by chain, side view.
PDB entry 2gwa coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Ligand bridging of the DNA Holliday junction: molecular recognition of a stacked-X four-way junction by a small molecule.
Brogden AL, Hopcroft NH, Searcey M, Cardin CJ
Angew Chem Int Ed Engl 46 3850-4 (2007)
PMID: 17477457

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115312 (preferred)
Entry contents:
1 distinct DNA molecule
Macromolecule:
5'-D(*Tp*Cp*Gp*Gp*Tp*Ap*Cp*Cp*Gp*A)-3' Chains: A, B
Molecule details ›
Chains: A, B
Length: 10 nucleotides
Theoretical weight: 3.05 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand A4C 1 x A4C
Ligand SPM 1 x SPM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: C2
Unit cell:
a: 65.224Å b: 25.142Å c: 37.541Å
α: 90° β: 110.97° γ: 90°
R-values:
R R work R free
0.277 0.268 0.31
Expression system: Not provided

Quick links

Citations

4 review citations

DNA-binding drugs caught in action: the latest 3D pictures of drug-DNA complexes.
Boer et al. (2009)
3 more

9 mentions without citation

Structure-based DNA-targeting strategies with small molecule ligands for drug discovery.
Sheng et al. (2013)
8 more